Perform true CRISPR gene editing in your classroom laboratory! CRISPR gene editing is revolutionising scientific research. Now your students can do real CRISPR-Cas9 gene editing in the classroom using Bio-Rad's educational Out of the Blue CRISPR Kit. Use CRISPR-Cas9 technology to introduce a stop codon in the lacZ gene in the E. coli chromosome, then see results easily with blue-white screening!

Where other educational kits merely showcase the cutting function of CRISPR-Cas9, the Bio-Rad Out of the Blue Gene Editing Kit demonstrates the need for both cutting and repair in gene editing. Students utilise CRISPR-Cas9 alongside homology-directed repair (HDR) to both cut and insert a stop codon into the chromosomal lacZ gene of E. coli. They can then use PCR to confirm that an edit was made. The carefully designed bacterial strain and plasmids in the Out of the Blue Gene Editing Kit allow students to safely explore and learn about the components of CRISPR gene editing, all with minimal equipment required. Clearly visible results allow students to see what happens if the Cas9 enzyme does not have a guide RNA to direct a cut, and students also witness the vital role of DNA repair machinery in the gene editing process. In the time it takes to perform a bacterial heat shock transformation, students perform true CRISPR-Cas9 genome editing to change a gene in a living cell, as is done in real-world applications of the technology!

Hands-on CRISPR gene editing - students get direct experience with this powerful technology
Safety - familiar, commonly used reagents and non-pathogenic bacteria generate a phenotype typical of molecular biology; instructions for safe disposal are also included
Accessibility - does not require specialiSed equipment or techniques
Visible phenotype - results can be observed with the naked eye in as little as 22 hr
More than just a cut, more than a knockout - use CRISPR-Cas9 technology to cut and introduce a stop codon into the chromosomal lacZ gene of E. coli
For educational use only
Each kit contains enough materials for 8 student workstations (2 to 4 students per workstation)
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